Relationship of reactive oxygen species levels in day 3 culture media to the outcome of in vitro fertilization/intracytoplasmic sperm injection cycles.
نویسندگان
چکیده
OBJECTIVE To examine the relationship of early human embryonic development parameters to day 3 reactive oxygen species (D-3 ROS) levels in culture media. DESIGN Prospective study. SETTING Tertiary care hospital. PATIENT(S) Patients were undergoing IVF (n=92; 36 with intracytoplasmic sperm injection [ICSI]). INTERVENTION(S) The D-3 ROS levels in sample and control of each embryo culture dish were measured by the chemiluminescence method using a luminol probe. MAIN OUTCOME MEASURE(S) Embryo quality (days 3 and 5) and pregnancy rates (PR). RESULT(S) The D-3 ROS level was significantly lower in pregnant cycles 26.8±13.9×10(6) cpm (counted photon per minute) versus nonpregnant cycles 66.4±39.4×10(6) cpm. This relationship was maintained when the cycles were stratified to conventional IVF (27.1±14.95 vs. 67.0±39.9×10(6) cpm) or ICSI (25.6±12.75 vs. 65.5±39.7×10(6) cpm). After controlling for all variables, D-3 ROS levels were negatively correlated with blastocyst development rate as well as PR. Odds ratio (OR) (95% confidence interval [CI]) of clinical pregnancy corresponding to a 10×10(6) cpm increase in D-3 ROS was 0.47 (0.30-0.74) for ICSI and 0.56 (0.37-0.85) for IVF. CONCLUSION(S) During extended in vitro culture, ROS generated in culture media by day 3 may be an important biochemical marker for blastulation. An increase of 10 units in D-3 ROS may decrease the clinical pregnancy by 41%.
منابع مشابه
Differential growth of human embryos in vitro: role of reactive oxygen species.
OBJECTIVE To examine the relationship of early human embryonic development with the level of reactive oxygen species (ROS) in the culture media on the first day (day 1 ROS) after insemination. DESIGN A prospective study. SETTING Patients undergoing assisted reproduction in a teaching hospital. PATIENT(S) Patients undergoing conventional IVF (n = 104; 115 cycles) and intracytoplasmic sperm...
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عنوان ژورنال:
- Fertility and sterility
دوره 94 6 شماره
صفحات -
تاریخ انتشار 2010